Supplementary MaterialsFigure S1: Schematics of CT scanning and mechanical testing setup. callus; and (D) 10 magnification of the box from (C). The fracture callus consisted of mostly fibroblast cells and chondrocytes in each case.(TIF) pone.0063857.s003.tif (4.7M) GUID:?C74D6D6E-1578-4E9E-BF59-6FE6804D274F Figure S4: H&E of fracture calluses at day 21 PF. (A) 4 magnification of wild-type callus; (B) 10 magnification of box from (A); (C) 4 magnification of Pten mutant callus; and (D) 10 magnification of box from (C). Woven bone had replaced the cartilage matrix in each case.(TIF) pone.0063857.s004.tif (4.8M) GUID:?27794FF3-C621-4E67-BB83-7042B3F02E73 Figure S5: H&E of fracture calluses at day 28 PF. (A) 4 magnification of wild-type callus; (B) 10 magnification of box from (A); (C) 4 magnification of Pten mutant callus; and (D) 10 magnification of box from (C). The callus consists of mostly woven bone in each case.(TIF) pone.0063857.s005.tif (4.4M) GUID:?D49D1CFA-C783-4A27-96A1-A62F305C0C8D Figure S6: Pten IHC of fracture calluses at day 7 AG-1478 ic50 PF. (A) 10 magnification of wild-type callus; (B) 20 magnification of box from (A); (C) 10 magnification of Pten mutant callus; (D) 20 magnification of box from (C). Pten was expressed at a similar level in each case.(TIF) pone.0063857.s006.tif (2.7M) GUID:?B402ABA8-84D3-4664-9615-D0D236E4696D Figure S7: Pten IHC of fracture calluses at day 14 PF. (A) 10 magnification of wild-type callus; (B) 20 magnification of box from (A); (C) 10 magnification of Pten mutant callus; (D) 20 magnification of box from (C). Pten was expressed at a higher level in the bone lining cells in the wildtype animals.(TIF) pone.0063857.s007.tif (3.3M) GUID:?EA81C6C3-DA92-4DF2-B4B2-ACBE943A4CC8 Figure S8: AG-1478 ic50 Pten IHC of fracture calluses at day 21 PF. (A) 10 magnification of wild-type callus; (B) 20 magnification of box from (A); (C) 10 magnification of Pten mutant callus; (D) 20 magnification of box from (C). Pten was expressed at a higher level in the bone lining cells in the wildtype animals.(TIF) pone.0063857.s008.tif (3.1M) GUID:?8BA5AFF5-C87A-4CB2-B472-D56972B9338A Figure S9: Pten IHC of fracture calluses at day 28 PF. (A) 10 magnification of wild-type callus; (B) 20 magnification of box from (A); (C) 10 magnification of Pten mutant callus; (D) 20 magnification of box from (C). Pten was expressed at a higher level in the bone lining cells in the wildtype animals.(TIF) pone.0063857.s009.tif (3.5M) GUID:?EB3BF3DA-A040-4C96-862E-9C680658CAC9 Figure S10: p-Akt IHC of fracture calluses at day 14 PF. (A) 10 magnification of wild-type callus; (B) 20 magnification of box from (A); (C) 10 magnification of Pten mutant callus; (D) 20 magnification of box from (C). p-Akt was expressed at a similar level in each case.(TIF) pone.0063857.s010.tif (2.8M) GUID:?FADE0768-2CFF-4B06-A9B5-C8FCE186A26B Figure PRKCB2 S11: p-Akt IHC of fracture calluses at day 21 PF. (A) 10 magnification of wild-type callus; (B) 20 magnification of box from (A); (C) 10 magnification of Pten mutant callus; (D) 20 magnification of box from (C). p-Akt was expressed at a similar level in each case.(TIF) pone.0063857.s011.tif (3.1M) GUID:?72634C43-1867-4EEA-B82E-1CE0D9BF0938 Figure S12: p-S6 IHC of fracture calluses at day 14 PF(A) 10 magnification of wild-type callus; (B) 20 magnification of box from (A); AG-1478 ic50 (C) 10 magnification of Pten mutant callus; (D) 20 magnification of box from (C). p-S6 was expressed at a similar level in each case.(TIF) pone.0063857.s012.tif (2.7M) GUID:?E4A0D89C-6306-4610-A826-1820EC0576F0 Figure S13: p-S6 AG-1478 ic50 IHC of fracture calluses at day 21 PF. (A) 10 magnification of wild-type callus; (B) 20 magnification of box from (A); (C) 10 magnification of Pten mutant callus; (D) 20 magnification of box from (C). p-S6 was expressed at a similar level in each case.(TIF) pone.0063857.s013.tif (3.4M) GUID:?A3FE8816-FF08-4DBE-9169-FF0F497FDB39 Figure S14: TRAP stain of fracture calluses at day 21 PF. (A) 10 magnification of wild-type callus; (B) 20 magnification of box from (A); (C) 10 magnification of Pten mutant callus; (D) 20 magnification of box from (C). TRAP staining was more intense in the mutant group.(TIF) pone.0063857.s014.tif (4.5M) GUID:?72C0D89B-F19A-499C-AF3D-9D479F66AE68 Document S1: Supplemental methods. (DOCX) pone.0063857.s015.docx (23K).