File names according to figures panels names. peerj-10-12751-s016.rar (59K) DOI:?10.7717/peerj.12751/supp-16 Supplemental Information 17: Dataset utilized for calculations in Fig. segment. peerj-10-12751-s003.pdf (494K) DOI:?10.7717/peerj.12751/supp-3 Supplemental Information 4: Absence of the NP CTD expression in the BL21[DE3]/pHYP-NP-CTD strain SDS-PAGE analysis of total E.coli proteins, 2 h induction by 1 mM IPTG, 30 oC, total proteins (induced), soluble and insoluble protein fractions. Cl.5, cl. 7Ctransformation of the BL21[DE3] cells by two numerous clones of the pHYP-NP-CTD plasmid. peerj-10-12751-s004.pdf (279K) DOI:?10.7717/peerj.12751/supp-4 Supplemental Information 5: Natural data of the ESI-MS analysis of the full-length NP preparation peerj-10-12751-s005.pdf (183K) DOI:?10.7717/peerj.12751/supp-5 Supplemental Information 6: Raw data of the ESI-MS analysis of the NTD preparation peerj-10-12751-s006.pdf (196K) DOI:?10.7717/peerj.12751/supp-6 Supplemental Information 7: Chromatography traces of the NP and NP-RNA protein preparations size exclusion chromatography analysis, dual wavelength optical detection (A, B) PBS as the mobile phase; (C, D) 300 mM BSc5371 NaCl, 20 mM sodium phosphate pH 7.5, 100 mM imidazole-HCl mobile phase; (E, F) 2 M NaCl, 20 mM sodium phosphate pH 7.5, 100 mM imidazole as the mobile phase. (C, D) are same to the Figs. 2E ?2F2F and are shown here for ease of the direct visual analysis. peerj-10-12751-s007.pdf (2.0M) DOI:?10.7717/peerj.12751/supp-7 Supplemental Information 8: Chromatography traces of the NP and NTD protein preparations size exclusion chromatography analysis, MALS and 280 nm detection (A) NP; (B) NTD. Molar masses, determined by the MALS detector, are shown on left side of peaks, molecular masses, determined by the calibration curve interpolation, are shown above peaks. peerj-10-12751-s008.pdf (242K) DOI:?10.7717/peerj.12751/supp-8 Supplemental Information 9: Size distribution by dynamic light scattering for NP variants (A, B) particle size distribution by intensity and volume for the BSc5371 real NP antigen. (C, D) RNase ACtreated NP, no 2 M NaCl wash. (E, F) Rnase ACuntreated NP, with 2 M NaCl wash. (G, H) NP-RNA. peerj-10-12751-s009.pdf (533K) DOI:?10.7717/peerj.12751/supp-9 Supplemental Information 10: Size distribution by dynamic light scattering for NTD variants (A, B) particle size distribution by intensity and volume for the real NTD antigen. (C, D) RNase ACtreated NTD, no 2 M NaCl wash. (E, F) Rnase ACuntreated NTD, with 2 M NaCl wash. (G, H) NTD-RNA. peerj-10-12751-s010.pdf (562K) DOI:?10.7717/peerj.12751/supp-10 Supplemental Information 11: Chromatography traces of the NTD and NTD-RNA protein preparations size exclusion chromatography analysis, dual wavelength optical detection (A, B) PBS as the mobile phase; (C, D) 300 mM NaCl, 20 mM sodium phosphate pH 7.5, 100 mM imidazole-HCl mobile phase; (E, F) – 2 M NaCl, 20 mM sodium phosphate pH 7.5, 100 mM imidazole as the mobile phase. peerj-10-12751-s011.pdf (2.0M) DOI:?10.7717/peerj.12751/supp-11 Supplemental Information 12: Antibody capture ELISA and dynamics of the assay specificity ratios for various purification methods, NP antigen (A) pure NP antigen, protein preparation and the ELISA test was independent from your test shown on Fig. 3. (B) NP protein antigen, treated by the RNase A and not treated by the on-column 2 M NaCl wash. (C) NP protein antigen, not treated by the RNase A and treated by the on-column 2 M NaCl wash. (D) NP-RNA antigen, protein preparation and the ELISA test was independent from your test shown on Fig. 3. (E) unfavorable control TrxA antigen, purified exactly as the NP antigen. (F) (OD+/OD-) for numerous serum samples dilutions, calculated as the ratios of OD readings for the PCR+ sample and the pre-COVID sample for the same sample dilutions. Blue lines – pooled PCR+ sera. Red lines – pooled pre-COVID sera. Statistical analysis by the or one-way ANOVA with the post-hoc Tukey-Kramer HSD test, three other antigen preparations as NP NP+-; NP NP-+; NP NP-RNA. peerj-10-12751-s012.pdf (381K) DOI:?10.7717/peerj.12751/supp-12 Supplemental Information 13: Antibody capture ELISA and dynamics of the assay specificity ratios for numerous purification methods, NTD antigen (A) real NTD antigen, protein preparation and the ELISA test was independent from your test shown on Fig. 3. (B) NTD protein antigen, treated Mouse monoclonal to ABCG2 by the RNase A and not treated by the on-column 2 M NaCl wash. (C) NTD protein antigen, not treated by the RNase A and treated by the on-column 2 M NaCl wash. (D) NTD-RNA antigen. (E) test specificity ratios (OD+/OD-) for numerous serum samples dilutions, calculated as the ratios of OD readings for the PCR+ sample and the pre-COVID sample at the same sample dilutions. Blue lines – pooled PCR+ sera. Red lines – pooled pre-COVID sera. Statistical analysis by the BSc5371 or one-way ANOVA with the post-hoc Tukey-Kramer HSD test, three other antigen preparations as NTD NTD+-; NTD NTD-+; NTD NTD-RNA. peerj-10-12751-s013.pdf (331K) DOI:?10.7717/peerj.12751/supp-13 Supplemental Information 14: Natural bitmap images As acquired by the flatbed scanner or video camera. peerj-10-12751-s014.zip (8.0M) DOI:?10.7717/peerj.12751/supp-14 Supplemental.