After that, the cells had been harvested simply by centrifugation at 7,000g and 4 C for 10 min, as well as the obtained pellets had been assessed simply by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) (12)
Heparanase 0 CommentsAfter that, the cells had been harvested simply by centrifugation at 7,000g and 4 C for 10 min, as well as the obtained pellets had been assessed simply by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) (12). Purification of DT386 recombinant proteins The expressed DT386 proteins was purified using Ni-NTA Purification program (Invitrogen, USA) while