Background and Seeks Early acknowledgement of prediabetes can lead to timely clinical interventions to prevent type 2 diabetes. quantify directly measured insulin resistance (IR). Isolated IFG was defined as fasting glucose ��100mg/dL and <140mg/dL at 2 hours with normal glucose tolerance during OGTT. Results Overall subject characteristics included median age 44 years 64 male 40 HCV-positive and 32% with isolated IFG. TGX-221 Factors associated with isolated IFG included subject age (OR 2.42 per decade 95 1.4 p=0.001) HCV illness (OR 4.0 95 1.71 p=0.002) and alanine aminotransferase (ALT) (OR 2.35 per doubling 95 1.46 p<0.0001). Multipredictor logistic regression analysis recognized ALT (OR 2.05 per doubling p=0.005 95 CI 1.24-3.40) and age (OR 2.20 per 10 years p=0.005 95 1.27 while factors independently associated with IFG. While HCV was TGX-221 associated with 4-collapse higher odds of IFG this entire effect was mediated by ALT. Conclusions We found strong evidence that liver inflammation is a risk element for prediabetes among Latinos with and without HCV. Among HCV-infected individuals early antiviral therapy could mitigate the effect of swelling and represent an important intervention to prevent diabetes with this at-risk populace. compiled list. Statistical significance was assessed at a p-value of <0.05 (2-sided) in all models. All analyses were performed using Stata version 12 statistical software Stata Corp LP College Station TX. Results Baseline Characteristics A total of 100 study participants who met the inclusion/exclusion criteria were analyzed (Table 1). The overall cohort characteristics were significant for median age 44 years 64 male 40 HCV-positive and 35% given birth to in the US. Overall TGX-221 32 subjects experienced isolated IFG and 68 experienced normal fasting glucose (NFG). The mean SSPG was 146 mg/dL and overall 26% of the total were insulin resistant (SSPG >180 mg/dL). A total of ten participants (10%) met criteria for metabolic syndrome based on presence of three or more clinical criteria including increased waist circumference elevated triglycerides reduced HDL cholesterol elevated blood pressure and elevated fasting glucose . Table 1 Characteristics of Subjects with (IFG) and without (NFG) Isolated Impaired Fasting Glucose Among HCV-infected participants the median ALT was 84.6 �� 53.0 U/L imply log10 HCV viral weight 5.8 �� 0.6 IU/mL 67 were HCV genotype 1 (19% genotype 2 14 genotype 3). Thirty four (85%) of HCV-infected individuals underwent liver biopsy with 67.6% having grade �� 2 inflammation 44.1% having stage �� 2 fibrosis and 26.5% with steatosis without evidence of steatohepatitis using the Ludwig-Batts rating system. Although liver biopsy is usually clinically indicated and standard of care for HCV-infected patients healthy controls were not subjected to invasive liver biopsy for this study. Rabbit Polyclonal to mGluR2/3. Characteristics of participants with and without isolated IFG were similar except subjects with IFG were older more likely to be US-born have HCV illness (63 vs 29%) have higher mean SSPG and be insulin resistant (34 vs 22%) TGX-221 and have higher ferritin levels. In addition a lower proportion of participants with IFG consumed a moderate amount of alcohol. TGX-221 Overall ALT levels were significantly higher (mean ALT 72 vs 39 U/L) for those with isolated IFG versus NFG (Number 1A). Although all participants experienced higher mean ALT levels in the establishing of IFG vs NFG when stratified by HCV status (95 vs 75 for HCV-infected and 35 vs 24 U/L for settings) statistical significance was reached in the HCV-uninfected group (p=0.002) (Number 1B). Number 1 Package plots of variations in alanine aminotransferase (ALT) levels Factors associated with IFG On single-predictor analysis (Table 2) subject age (OR 2.42 per decade 95 1.4 p=0.001) HCV illness (OR 4.00 95 1.71 p=0.002) ALT level (OR 2.35 per doubling 95 CI 1.46-3.77 p<0.0001) and ferritin level (1.05 95 1.03 p=0.027) were positively associated with isolated IFG. In addition metabolic syndrome (OR 2.33) and US birth were positively associated (OR 2.10) while moderate alcohol usage was negatively associated (OR 0.39) with IFG but these did not quite reach statistical significance. We further assessed whether the association of HCV with isolated IFG was mediated through liver inflammation as hypothesized using logistic modeling with and without ALT. The statistically significant four-fold higher odds of IFG for HCV-infected subjects.