Gene therapy is a promising technique for the treatment of various

Gene therapy is a promising technique for the treatment of various diseases. endocytosis and subsequent release from your endosomes further support the improved gene transfection effectiveness of the CeO2/DODAB vectors. CD 437 Besides CeO2/DODAB nanovectors could transfect genes without any sign of toxicity. Taken together this fresh nano-vector gets the potential to be utilized for gene delivery in biomedical applications. Gene therapy continues to be recognized as a promising technique to treat genetic disorders and cancers. Gene therapy involves the reparation of defective genes or the incorporation of new functional genes into the cells1 2 However the negatively charged genes cannot traverse the negatively charged cell membrane effectively without the assistance of gene delivery vectors. Gene delivery vectors include both viral3 4 5 and non-viral systems6 7 8 Although viral vectors show high transaction efficiency they are limited in terms of DNA packaging and are also hazardous to humans9. Therefore nonviral vectors have gained prominence because they are largely biocompatible easily functionalized and varied structurally and have the potential to carry diverse genetic materials into living cells10. Non-viral gene delivery vectors have to overcome three important barriers during gene delivery: (1) DNA entry across the cell membrane (2) CD 437 protection of DNA bound to the vectors and subsequent release of DNA and (3) DNA entry into the nucleus. Therefore development of minimally CD 437 toxic and highly efficient non-viral gene delivery vectors is the most challenging undertaking in the field of gene therapy11. In recent years inorganic nanoparticle-based gene delivery vectors have gained the attention of researchers due to their unique physical and chemical properties12. Several types of inorganic nanoparticles can form stable complexes with DNA and deliver it into living cells. These include silica nanoparticles13 14 quantum dots15 Au nanoparticles16 17 18 carbon nanotubes19 20 hybrid nanoparticles21 etc. Herein for the first time we introduce nanoceria (CeO2) as a non-viral gene delivery vector. Nanoceria is well known for its excellent antioxidant activity22 23 24 25 26 27 Nanoceria is a reported mimic for superoxide dismutase (SOD) with catalytic efficiency surpassing that of SOD itself28 29 Nanoceria has the ability to alter its valence state (between Ce3+ and Ce4+) and create oxygen defects on its surface. The catalytic activity of nanoceria is derived directly from this property30 31 Therefore it is quite reasonable to use nanoceria as a gene delivery vector due to its biocompatible nature. Liu transfection efficiency and cytocompatibility of the CD 437 nanoparticles and intracellular distribution of the nanoparticle-DNA complexes (vi) investigated the intracellular uptake pathways of the nanoparticle-DNA complexes and (vii) evaluated the transfection efficiency CD 437 and biocompatibility of the nanoparticles. Results Preparation and characterization of nanoceria (CeO2) DODAB-modified nanoceria (CeO2/DODAB) and CeO2/DODAB-pDNA complexes In the present study nanoceria (CeO2) was prepared by simply refluxing ammonium cerium(IV) nitrate and urea according to the method of Tsai39. The synthesized CeO2 was characterized by energy dispersive spectroscopy (EDS) and Fourier transform infrared (FTIR) spectroscopic analyses. The EDS spectrum showed characteristic peaks of Ce and O and was devoid of any impurity peaks (Fig. 1a). However a Cu peak arising from the TEM grid and a Si peak from the detector were observed. The chemical nature of CeO2 was also verified from the FTIR spectrum which showed a strong absorption band at 500?cm?1 due to the Ce-O stretching vibration (Fig. 1b). Infrared absorption rings were Rabbit Polyclonal to BAGE3. noticed at 3385?cm?1 1545 and 1340?cm?1 CD 437 because of drinking water and CO2 substances adsorbed for the nanoparticle surface area (Fig. 1b). After confirming the formation of CeO2 CeO2/DODAB was made by basically blending CeO2 and DODAB inside a 1:2 mole percentage. The quantity of DODAB (6.30%) bound to the nanoceria surface area was calculated from the quantity of nitrogen within CeO2/DODAB (Supplementary Desk 1). The optical absorbance of synthesized CeO2 was examined by acquisition of the UV range which showed a definite absorption music group at 295?nm and was without impurity peaks (Fig. 1c). Yet in the entire case of CeO2/DODAB the absorption band appeared at 315?nm (Fig. 1c). The CeO2/DODAB-pDNA complicated (CeO2/DODAB to pEGFP-N1 mass percentage?=?30) showed a wide music group spanning 315-320?nm (Fig. 1c)..