A mycosed planthopper, Berg (Hemiptera: Cixiidae), and two psocids, sp. Delphacidae)

A mycosed planthopper, Berg (Hemiptera: Cixiidae), and two psocids, sp. Delphacidae) after 10 days. Therefore, based on the morphology of the isolated fungi, their ribosomal internal transcribed spacer sequence, and their ability to parasite bugs, we conclude the fungi isolated belong to the genus and might possess biotechnological potential. (Brefeld) Batko, SB 203580 have relatively wide ones (Wraight et al. 2007). The most common genera include is one of the most abundant and important entomogenous fungi (Liang et al. 2005) and might play an important part in the control of pest bugs in nature (Evans 1974, 1982). The genus was erected by Patouillard (1892), based on the type varieties Pat., which he explained from a specimen collected on a beetle in Ecuador. Minier and Brady (1980) divided this entomogenous genus in two sections (Synnematous and Mononematous) based on the presence or absence of synnemata. Most varieties of are synnematous, a few are mononematous, and some others occasionally create synnemata. Many users are believed to be anamorphs of teleomorphs within the genera and (Petch 1935; Mains 1951; Kendrick and Carmichael 1973; Hywel-Jones 1995a, b, 1997; ). They include more than 90 varieties that infect and parasitize a variety of invertebrate varieties, such as mites, nematodes, and bugs, many of which are economically important pests (Seifert and Boulay 2004). Rabbit polyclonal to TLE4. Although F.E. Fisher, a parasite of citrus rust mite, was used to make commercial formulations in the USA, the use of representatives of this genus to control pests is limited because many of these pathogens are fastidious organisms that require specific conditions to grow (Boucias et al. 2007a). In Argentina, there is increasing concern concerning the use of chemicals to control bugs, consequently many labs and companies are developing natural products to control pests. Regarding this development, it is important to have a wide array of characterized entomopathogenic fungi. However, little is known about the presence and fungal-host in- teraction of varieties of in Argentina. There is only one statement by Toledo et al. (2008) describing the presence of Speare (Ascomycota: Hypocreales: Clavicipitaceae) and Petch infecting (Eschscholtz) (Dermaptera: Forficulidae) and (Banks) (Psocodea: Ectopsocidae), respectively. As part of a system aimed at developing biocontrol tools, entomopathogenic fungi are becoming isolated from different bugs. Here, we statement the isolation of associates of the genus on naturally infected bugs belonging to the orders Hemiptera and Psocodea. We recognized and characterized them by means of morphological and molecular heroes. Additionally we confirmed that both strains infected and parasitized Fennah (Hemiptera: Delphacidae), the major vector of disease, a severe endemic disease in corn plants in Argentina. Materials and Methods Isolation and preservation of fungal pathogens Between February and SB 203580 September 2007, mycosed adults of Berg (Hemiptera: Cixiidae) and sp. (Psocodea: Pseudocaeciliidae), SB 203580 and a nymphal sp. (Psocodea: Ectopsocidae) were collected from rice, L. (Poaceae), from Los Hornos, Buenos Aires, Argentina (34 52 S, 57 58 W), and from Ait. (Oleaceae) from La Plata, Buenos Aires, Argentina (34 54 S, 57 56 W), respectively. Infected bugs were placed in sterile plastic containers and were taken to the laboratory to isolate fungi. These bugs were so badly damaged that some essential elements for his or her identification in the varieties level were lacking. Fungal SB 203580 ethnicities were from monosporic isolates in the manner explained by Lecuona (1996) and incubated on Sabouraud dextrose agar +1% candida draw out (SDAY 1%) at 26 C in the darkness for 15 days. Mycosed planthoppers were deposited as herbarium material in the Mycological Collection of the Institute of Botany Carlos Spegazzini (LPSC, La Plata, Buenos Aires, Argentina) under the accession quantity LPSC 47786. Subcultures of the fungi, isolated from planthoppers and psocids, were deposited in the USDA-ARS Collection of Entomopathogenic Fungal Ethnicities under the accession figures ARSEF 8378 for planthoppers, and ARSEF 8677, 8678, and 8679 for psocids. Recognition and morphological characterization of fungal pathogens Fungal constructions such us conidia and conidiogenous cells from deceased bugs were measured to enable specific recognition. Mycelia were mounted in lactophenol cotton blue (0.01% w/v) and observed having a Wild M20 microscope. Infected bugs were photographed using a Wild M5 stereo microscope fitted having a Sony Cyber-shot DSCW100 digital camera (www.sony.com). Fungal preparations were photographed using a Nikon YS2-H microscope (www.nikon.com) equipped with a Nikon D40 digital camera. Monosporic isolates cultured on SDAY 1% were incubated at 26 C in darkness for 21 days. Growth rates and aspects of the colony were recorded. Growth.